Microbial biofilms are significant in a variety of settings including the human microbiome, infectious disease, industrial processes, and environmental remediation. Due to the ubiquitous nature of biofilms, there is a great interest in understanding cellular activities within the biofilm matrix. Within a biofilm cells are able to better withstand environmental stress, experience increased horizontal gene transfer, and live longer. The purpose of this research is to grow pseudomonas sp. strain ADP in biofilm culture and examine the chemical and physical characteristics the microbe undertakes within the sticky extracellular matrix. ADP is the organism of choice because of its ability to degrade atrazine and because there is limited information about this organism in biofilms. Cells are grown using atrazine as the sole nitrogen source in drip and flow cell reactors. Varying shear stress and time lapse will give insight into biofilm formation. As a positive control, Escherichia coli are grown in a similar manner. Chemical analysis is performed using Raman spectroscopy and light microscopy to examine cellular distribution. Success of this research will aid in the building of the microbial Raman library and in the development of non-invasive techniques to examine both internal composition and spatial distribution of compounds present in biofilms.