Modern drug discovery is a time-consuming process, particularly when searching for allosteric effectors that change a target’s activity. Two-Dimensional Infrared Spectroscopy (2D IR) is a powerful tool capable of answering the question “Does binding of this effector change vibrational activity at the active site?”, which is a likely indicator of allosteric effectors. My research focuses on pushing limits of detection of 2D IR to enable it as a screening tool for allosteric effectors.