Dopamine is a tightly regulated molecule and the disruption of its homeostasis is linked to Parkinson’s disease. Parkinson’s is not diagnosed until after mass neuronal death, making earlier diagnostic techniques imperative. Disruption of dopamine pathways likely begins decades prior to noticeable cell death. This dissertation work describes the discovery and examination of a novel, dopamine-derived biomarker that is produced after dopamine homeostasis disruption and exposure to Parkinson’s-linked environmental neurotoxicants.