Enzymes are biological catalysts, which are important for maintaining the cellular activity by increasing the rate of biochemical conversion. I am studying-kinetics, mechanisms and dynamics of the enzyme Thymidylate Synthase (TSase), which is one of the chemotherapeutic drug targets. Despite many therapeutic advantages, current drugs targeting TSase are highly toxic and lead to resistance in tumor cells. Our research might find novel selective classes of TSase inhibitors by re-examination the mechanistic and kinetics features of TSase.