Mentor
Dr. Tara Smith
Participation year
2009
Project title

Molecular Epidemiology of Methicillin Susceptible Staphyloccocus Aureus (MSSA) in Iowa Pig Farms

Abstract

Staphylococcus aureus is a nosocomial pathogen that causes several diseases, including endocarditis, pneumonia, toxic-shock syndrome and food poisoning. It has been documented previously by our research group the presence of methicillin susceptible Staphyloccous aureus (MSSA) in U.S. swine and in swine farmers. We hypothesized that the MSSA found in swine and swine farmers would not present the pvl (Panton-Valentine leukocidin) gene, which is more common in methicillin resistant Staphylococcus aureus. We also hypothesized that we would be able to find different bacterial strains between the different samples of MSSA through bacterial strain typing, specifically of the spa gene. This method let us distinguish epidemiologically related or clonal isolates from unrelated isolates. It also has become an important clinical tool to investigate outbreaks and transmission of the gram positive bacteria. We found that all isolates were negative for the pvl gene and the first evidence of what can be interpreted as common strains between the isolates of the MSSA with almost no variability between them.

Sheila  Berrios Nazario
Education
Univeristy of Puerto Rico at Humacao